With most non-league sides not in action until Sunday, opportunities for upsets were at a premium on Saturday, when the most surprising result was Chesterfield’s 1-0 win at League One high-flyers MK Dons.

Karl Robinson’s side had won five and drawn one of their previous six games but were knocked out by Armand Gnanduillet’s 53rd-minute header for Chesterfield.

One non-league side definitely through are Wrexham of the Conference Premier, who beat Ryman League Premier team Maidstone United 3-1. Andy Bishop was the Welsh side’s match-winner, scoring twice.

Any hopes Plymouth had of causing a shock at Sheffield United disappeared in an eventful opening to the second half in which the League Two side missed a penalty and their opponents scored two. Argyle won a spot-kick early in the second half when Lewis Alessandra was fouled by Jay McEveley, but Reuben Reid sent the resulting penalty high over the bar.

They were punished when United won penalties in the 55th and 62nd minutes – for fouls by Ben Purrington on Marc McNulty, and Peter Hartley on Jamie Murphy respectively – that were converted by Jose Baxter. McNulty made it 3-0 in the closing stages.

Accrington of League Two are still in the hat after a 1-1 home draw with the League One strugglers Yeovil, while the Doncaster manager, Paul Dickov, enjoyed a winning return to his former club Oldham. Genseric Kusunga’s own goal in the 86th minute settled a tight encounter.